Development of Tn5 Mutants of Cicer-Rhizobium Strains Tolerant to Nitrate under Symbiotic Condition |
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Authors: | Debasis Chattopadhyay M L Lodha |
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Institution: | 1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012, India
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Abstract: | Transposon Tn5 mutants of two Cicer-Rhizobium strains, G36-84 and F-75 tolerant to nitrate under symbiotic condition, have been developed. Suicide vector pGS9 was used as a source of Tn5 for insertion mutagenesis of Rhizobium. The mutants so developed were screened in the presence of nitrate under symbiotic condition for nodule formation (Nod) and N2-fixation (Fix) ability. We could get some mutants, which were Nod+ Fix+ at 1mM and 2 mM nitrate, from the parental strains which were Nod- at 1 mM nitrate. |
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