拟南芥角质层发育突变体中WBC11基因的分析与鉴定

从拟南芥(Arabidopsis thaliana L.)突变体库中筛选到一个发育突变体ku7fy1,其突变表型为叶片狭长,生长缓慢。该研究利用图位克隆技术和候选基因测序鉴定出ku7fy1角质层发育基因(white-brown complex11,WBC11)有一个点突变。对该突变体cDNA测序结果显示,WBC11基因的突变导致其第7个内含子在形成成熟mRNA时无法被正常剪切,使该突变体内WBC11的mRNA大量降解并在翻译时提前引入终止密码子。甲苯胺蓝染色实验显示,突变体叶片表面角质层有缺陷;遗传互补实验进一步证明,突变体ku7fy1中的突变基因是WBC11,ku7fy1表型是由WBC11突变造成的。

Analysis and Identification of WBC11 Gene in a Cuticle Development Mutant of Arabidopsis thaliana

A development mutant ku7fy1 was obtained in mutant screening in Arabidopsis thaliana.It has a phenotype of narrow leaf and stunted growth.In this study,using the map-based cloning strategy and candidate gene sequencing we identified a point mutation in the WBC11(white-brown complex11) gene of ku7fy1.cDNA sequencing result of the mutant indicated that the mutation resulted in the detention of the 7th intron in the mature mRNA,which not only caused degradation of the mRNA but also led to premature translational termination.Toluidine blue staining suggested that its leaf cuticular had a defect.Genetic complementation experiment further confirmed that the mutant gene was WBC11.