Construction and Screening of Barley Genomic Library with a B1-Hordein Gene Probe |
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| Authors: | Aruna Tyagi I. M. Santha S. L. Mehta |
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| Affiliation: | 1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012, India
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| Abstract: | Barley genomic library from cv NP113 was made in a replacement vector EMBL-3. The titre was found to be 1.25 x 106 per μg of genomic DNA. The recombinants were screened using a B1-hordein DNA probe. One clone contained a positively hybridizing 4 kb fragment. |
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