Isolation,structural and toxicological characterization of three new mycotoxins produced by the fungusAureobasidium pullulans

3 substances, B₁, B₂, and E₁ were isolated from culture medium extracts ofAureobasidium pullulans by reversed phase liquid chromatography and subsequent liquid chromatographic purification steps on silica gel. The 3 compounds inhibited the metabolism ofSaccharomyces cerevisiae and showed toxic effects in the growth inhibition test toEscherichia coli andBacillus subtilis. Elementary analysis and mass spectroscopical methods revealed sum formulas of C₂₃H₂₂O₆, C₂₂H₂₀O₆ and C₂₄H₂₈O₃ for B₁ B₂, and E₁ and molecular weights of 394, 380, and 364, respectively. Mass spectroscopical, UV-, IR-,¹³C-NMR, and¹H-NMR-spectroscopical investigations revealed polycyclic, non-aromatic compounds containing several carbonyl functions and double bonds and, most notably, spiroepoxy-functions, in the case of B₁ and B₂.