A Simple Method for the Purification of Organelle DNA of Plants |
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| Authors: | P. Nath A. P. Sane V. Bijola P. K. Trivedi J. Arora P. V. Sane |
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| Affiliation: | 1. Plant Molecular Biology and Biochemistry Laboratory, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India
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| Abstract: | We report here a simple procedure for the purification of the organelle DNA. Mitochondrial DNA from Sorghum and the chloroplast DNA from Populus and spinach were purified using this protocol. The method utilizes a quick centrifugation of the isolated organelle DNA through a two step CsCl density gradient for removal of small molecular weight nucleic acids which pose a major problem for getting clean restriction patterns. This method of purification can be adopted with any isolation procedure for organelle DNA. |
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