Effects of dissolved oxygen concentration and DO-stat feeding strategy on CoQ10 production with Rhizobium radiobacter |
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Authors: | Wu Zufang Du Guocheng Chen Jian |
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Institution: | (1) Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Southern Yangtze University, Wuxi, 214036 Jiangsu Province, P.R. China;(2) Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211 Zhejiang province, P.R. China |
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Abstract: | The cell growth and CoQ10 (coenzyme Q10) formation of Rhizobium radiobacter WSH2601 were investigated in a 7-1 bioreactor under different dissolved oxygen (DO) concentrations. A maximal CoQ10 content (C/B) of 1.91 mg/g dry cell weight (DCW) and CoQ10 concentration of 32.1 mg/l were obtained at the appropriate DO concentration of 40% (of air saturation). High DO concentration was favourable to the cell growth of Rhizobium radiobacter WSH2601. In order to achieve the maximal yield of CoQ10 production, a new DO-stat feeding strategy was proposed, which significantly improved cell growth and CoQ10 formation. With this strategy, the maximal CoQ10 concentration and DCW reached 51.1 mg/l and 23.9 g/l, respectively, which were 67 and 44.8% higher than those obtained in the batch culture with DO concentration controlled. |
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Keywords: | CoQ10 dissolved oxygen DO-stat feeding strategy Rhizobium radiobacter |
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