| 离体缺血再灌注鼠心肌钙离子的变化 |
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| 引用本文： | 车成日,李香善,金基焕,王明勇,李素香,李丹. 离体缺血再灌注鼠心肌钙离子的变化[J]. 生物化学与生物物理进展, 1994, 21(1): 73-76 |
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| 作者姓名： | 车成日 李香善 金基焕 王明勇 李素香 李丹 |
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| 作者单位： | 延边医学院附属医院胸心外科;延边医学院心血管研究室;延边医学院附属医院胸心外科;延边医学院附属医院胸心外科;延边医学院心血管研究室;延边医学院心血管研究室 |
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| 基金项目： | 国家自然科学基金资助项目分题. |
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| 摘 要： | 用液体闪烁计数法测定离体再灌注鼠心肌肌质网(SR)和线粒体(Mit)内 45Ca2+放射性强度(cpm),比较能量制剂ATP-MgCl2,活性氧自由基清除剂SOD和钙阻滞剂Verapamil对离体缺血再灌注鼠心肌细胞SR和Mit钙离子浓度的影响.结果表明,SR内ATp-MgCl2,SOD和Verapamil组 45Ca2+的cpm均高于对照组(P<0.0l或P<0.05),而Mit内均低于对照组(P<0.01).此三种药均能提高离体大鼠心肌细胞内SR 45Ca2+和降低Mit 45Ca2+积聚,从而保护了心肌细胞,防止缺血再灌注损伤.
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| 关 键 词： | 缺血－再灌注 心肌肌质网 线粒体 钙离子 |
| 收稿时间： | 1993-01-28 |
| 修稿时间： | 1993-04-08 |
The Change of Ca2+ in SR and Mit of Isolated Ischemnia-Reperfusion Rat Heart |
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| Che Chengri,Li Xiangshan,Jin Jihuan,Wang Mingyong,Li Suxiang and Li Dan. The Change of Ca2+ in SR and Mit of Isolated Ischemnia-Reperfusion Rat Heart[J]. Progress In Biochemistry and Biophysics, 1994, 21(1): 73-76 |
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| Authors: | Che Chengri Li Xiangshan Jin Jihuan Wang Mingyong Li Suxiang Li Dan |
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| Affiliation: | Department of Thoracic and Cardiovascular Surgery, Affliated Hospital, Yian Bian Medical College;Department of Thoracic and Cardiovascular Surgery, Affliated Hospital, Yian Bian Medical College;Department of Thoracic and Cardiovascular Surgery, Affliated Hospital, Yian Bian Medical College;Department of Thoracic and Cardiovascular Surgery, Affliated Hospital, Yian Bian Medical College;Department of Thoracic and Cardiovascular Surgery, Affliated Hospital, Yian Bian Medical College;Department of Thoracic and Cardiovascular Surgery, Affliated Hospital, Yian Bian Medical College |
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| Abstract: | The strength of radiation of 45Ca2+ in sarcoplasmic reticulum vesicle (SR) and mitochondria (Mit) was measured by liguid scintillation counting method. Comparing the effects of ATP - MgCl2 . SOD and verapamil on the 45Ca2+ concentration in SR and Mit which prepared from isolated ischemia reperfused rat hearts. The result showed that the 45Ca2+ cpm of SR of the three groups were higher than that of control, while the 45Ca2+ cpm of Mit of the three groups were lower than that of control. These results suggested that the three kinds of reagent could protect reperfusion injury of heart cell through enhance 45Ca2+ storage in SR and inhibitting the 45Ca2+ accumulation in Mit. |
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| Keywords: | ischemia-reperfusion sarcoplasmic reticulum vesicle mitochondria calcium |
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