肠道微生物菌株资源库的构建与应用开发

肠道微生物组在组成和功能上都具有极高的复杂性,大量基于免培养的微生物组学研究表明肠道菌群失调与多种疾病都存在密切关联,肠道菌群的稳态与宿主健康密切相关已是共识.同时,越来越多的研究者认识到,可培养微生物菌株资源是肠道微生物组研究从关联分析向功能验证、机理解析和应用开发方向深入发展的基础和保障.本文主要对近年来完成的一些...

Gut Microbial BioBanks: Construction and Applications

The gut microbiome (GM) is extremely complicated in composition and function. Many culture-independent studies have demonstrated that the GM plays a pivotal role in host health and its imbalance has close relevance with various diseases. Meanwhile, increasing more researchers have realized that the culturable gut microbial biobanks are the resource foundations to facilitate the in-depth studies of GM by transition from meta-data based on relevance analysis to experimental validation of host-microbe interactions and GM-disease causality and further application in human health management. In this review, we summarize the recent progresses on large-scale gut microbial isolation and cultivation from different hosts, and provide a few examples for application of cultured microbial resources in high throughput sequencing data analyzing and in host-microbe interaction studies.