(Na++K+)-adenosinetriphosphatase in the brain of Shiverer (Shi/Shi) mice |
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| Authors: | Harold J Sheedlo George J Siegel Timothy J Desmond Stephen A Ernst |
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| Institution: | (1) Department of Neurology, University of Michigan, Neuroscience Laboratory Building, 1103 East Huron, 48109 Ann Arbor, Michigan;(2) Department of Anatomy and Cell Biology, University of Michigan, Neuroscience Laboratory Building, 1103 East Huron, 48109 Ann Arbor, Michigan |
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| Abstract: | The myelin-deficient Shiverer (Shi/Shi) mutant mouse may be a useful model in assessing the dependence of brain (Na++K+)-ATPase concentration and composition on myelin membrane formation. Brain microsomal membranes from age-matched control (+/+) and Shiverer (Shi/Shi) mice were fractionated by differential centrifugation and sucrose gradient sedimentation. No reduction in (Na++K+)-ATPase specific activity was measured in whole homogenates, high-and low-speed fractions or gradient fractions from brains of Shi/Shi mice as compared to those of +/+ mice. In addition, sodium dodecylsulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with antisera specific for mouse brain (Na++K+)-ATPase revealed no significant difference in catalytic subunit composition between fractions of +/+ and Shi/Shi brains. The similar results obtained for both +/+ and myelin-deficient Shi/Shi mice suggest that myelin contributes little to total brain (Na++K+)-ATPase. |
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| Keywords: | Shiverer myelin (Na++K+)-ATPase brain antibody mouse |
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