| 离子注入花生四烯酸产生菌诱变选育 |
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| 引用本文： | 姚建铭 王纪 王相勤 袁成凌 王文生 余增亮. 离子注入花生四烯酸产生菌诱变选育[J]. 生物工程学报, 2000, 16(4): 478-481 |
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| 作者姓名： | 姚建铭 王纪 王相勤 袁成凌 王文生 余增亮 |
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| 作者单位： | 中国科学院等离子体物理研究所离子束生物工程中心,合肥,230031 |
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| 基金项目： | 安徽省地方攻关项目支持(85-722-22-01)。 |
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| 摘 要： | 利用离子束注入生物技术对花生四烯酸产生菌(Mortierella alpina)进行诱变高产菌筛选。筛选到高产菌I49N18，该菌每升培养液可得生物量30.80g(约4%的含水量)，干菌体油脂含量为25.8%，其中花生四烯酸的含量占总.脂的45.37%。30L和250L发酵罐发酵试验，该高产菌的花生.四烯酸得率为4.0g/L。
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| 关 键 词： | 花生四烯酸，离子注入，筛选，发酵 |
| 文章编号： | 1000-3061(2000)04-0478-04 |
| 修稿时间： | 1999-07-05 |
Breeding of Arachidonic Acid Producting Strain by Ion Implantation |
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| YAO Jian-Ming,WANG Ji,WANG Xiang-Qin,YUAN Cheng-Ling,WANG Wen-Sheng,YU Zeng-Liang. Breeding of Arachidonic Acid Producting Strain by Ion Implantation[J]. Chinese journal of biotechnology, 2000, 16(4): 478-481 |
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| Authors: | YAO Jian-Ming WANG Ji WANG Xiang-Qin YUAN Cheng-Ling WANG Wen-Sheng YU Zeng-Liang |
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| Affiliation: | YAO Jian-Ming,WANG Ji,WANG Xiang-Qin,YUAN Cheng-Ling,WANG Wen-Sheng,YU Zeng-Liang;(Centre of Ion Beam Bioengineering, Institute of Plasma Physics, Academia Sinica, Hefei 230031) |
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| Abstract: | With ion implantation,a high-yield arachidonic acid producting strain(Mortierella alpina)I49-N18 was selected,whose biomass was 30.80g/L. The lipid component of biomass was 25.8%, in which AA content was 45.37%. The results showed that selected high-yield strain was steady on 250L fermentor,whose AA yield was 4.0g/L. |
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| Keywords: | Arachidonic acid ion implantation screening ferment |
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