Biological characteristics of plasmids of <Emphasis Type="Italic">Mesorhizobium huakuii</Emphasis> HN3015 from <Emphasis Type="Italic">Astragalus sinicus</Emphasis> |
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Authors: | Guoyuan Hu Youguo Li Junchu Zhou |
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Institution: | (1) State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China;(2) School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, 430073, China |
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Abstract: | A Mesorhizobium huakuii strain HN3015 was isolated from Astragalus sinicus in a rice-growing field of Southern China. Strain HN3015 contained three large plasmids. The three indigenous plasmids, named
as pMhHN3015a, pMhHN3015b and pMhHN3015c of M. huakuii HN3015, were, respectively, cured by Tn5-sacB insertion. The mutant strain HN3015-1 cured with its largest plasmid pMhHN3015c formed only white null nodules. Mutant HN3015-3
cured with its smallest plasmid pMhHN3015a could form pink effective nodules. However, mutant HN3015-2 cured of the second
largest plasmid pMhHN3015b lost nodulation ability. Furthermore, curing of pMhHN3015a had enhanced competitive nodulation
ability and symbiotic efficiency of HN3015-3. The results from acidity tolerance assays indicated that the three plasmids
in M. huakuii HN3015 had a positive control effect on acidity tolerance of HN3015, and all indigenous plasmids of M. huakuii HN3015 had a negative control effect on the alkali tolerance capacity of HN3015. Surprisingly, all plasmids in M. huakuii HN3015 had also a negative control effect on its growth rate. The results showed an interactive and functional complexity
of plasmids in strain HN3015. |
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Keywords: | Astragalus sinicus Mesorhizobium huakuii Indigenous plasmid Plasmid curing Plasmid interaction |
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