Extracellular catalase induces cyclooxygenase 2, interleukin 8, and stromelysin genes in primary human chondrocytes |
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Authors: | Litvinov Dmitry Turpaev Kyril |
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Affiliation: | Engelhardt Institute of Molecular Biology, the Russian Academy of Sciences, Vavilov street 32, Moscow 119991, Russia. |
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Abstract: | We investigated the expression of genes in response to exposure of primary human chondrocytes to extracellular catalase. The addition of catalase to culture medium caused a significant up-regulation of cyclooxygenase 2, interleukin 8, and stromelysin mRNA levels. Similar pattern of gene activation occurred in chondrocytes incubated with horseradish peroxidase. On the contrary, ebselen, a glutathione peroxidase mimetic agent, did not affect expression of catalase-inducible genes. Taken together, these observations imply that catalase action is mediated by its side peroxidase-like activity, rather than elimination of H2O2. Genistein suppressed catalase-mediated effects on gene expression. This finding implies that tyrosine kinases are implicated in underlying signaling pathway. |
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Keywords: | Catalase Peroxidase Chondrocytes Gene expression |
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