The importance of the fixation of colour,pattern and form in tropical Pseudocerotidae (Platyhelminthes,Polycladida) |
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Authors: | L. J. Newman L. R. G. Cannon |
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Affiliation: | (1) Worms, Queensland Museum, South Brisbane, P.O. Box 3300, 4101 Queensland, Australia;(2) Present address: Zoology Department, University of Queensland, St. Lucia, 4072 Queensland, Australia |
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Abstract: | At last a fixation method that ensures tropical pseudocerotid polyclads are fixed flat, preserved for histological preparation and which also retains their colour pattern has been developed. FCA-PGPP (Formaldehyde Calcium Acetate-Propylene Glycol, Propylene Phenoxetol) fixative is frozen and worms are coaxed onto filter paper which is then laid on the frozen fixative. As a consequence, over 230 species have been documented from the southern Great Barrier Reef and eastern Papua New Guinea (Newman & Cannon, 1994; in press). It was determined that species diagnoses need to be based on colour pattern, general morphology of living animals and serial reconstructions of the male anatomy.Christensen Research Institute Contribution No. 82 |
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Keywords: | Polycladida Pseudocerotidae Pseudoceros fixation preservation colour pattern |
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