Identification of <Emphasis Type="Italic">mokB</Emphasis> involved in monacolin K biosynthesis in <Emphasis Type="Italic">Monascus pilosus</Emphasis> |
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Authors: | Kanae Sakai Hiroshi Kinoshita Takuya Nihira |
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Institution: | (1) International Center for Biotechnology, Osaka University, 2-1, Yamadaoka, Suita Osaka, 565-0871, Japan;(2) MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand; |
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Abstract: | Monacolin K (MK), which is widely used as an antihypercholesterolemia medicine, is produced as a fungal secondary metabolite
through the polyketide pathway. The MK biosynthetic gene cluster proposed for Monascus pilosus BCRC38072 was also identified in M. pilosus NBRC4480. The mokB gene, located at the end of the putative gene cluster and possibly encoding polyketide synthase, was disrupted. The mokB disruptant did not produce MK, but accumulated an intermediate that was confirmed to be monacolin J, indicating that mokB encodes the polyketide synthase responsible for the biosynthesis of side-chain diketide moiety. |
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