Cloning of the Microcystis aeruginosa M228 lectin (MAL) gene |
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Authors: | Jimbo M Yamaguchi M Muramoto K Kamiya H |
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Institution: | Department of Marine Biochemistry, Kitasato University, Sanriku, Iwate, 022-0101, Japan. mjimbo@kitasato-u.ac.jp |
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Abstract: | We have cloned and characterized the gene encoding Microcystis aeruginosa (strain M228) lectin (MAL). The gene contains 1551 nucleotides and an open reading frame for a protein of 517 amino acids with a predicted molecular weight of 55,159 Da. The carboxy-terminal region of MAL has three tandemly repeated homologous domains composed of 61 amino acids. These regions show similarity to the corresponding regions of the alpha-amylase of Clostridium beijerinckii (23% identity). The mal gene lies adjacent to an ORF that display homology to cytochrome P-450 and polyketide synthase. Southern hybridization showed that the genomic DNA of the strain M228 contained, in addition to MAL gene (mal), at least two other mal like gene. |
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