Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality |
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Authors: | Lei Ma Dae-Hyun Kim Eun-Ju Jung Woo-Jin Lee Ju-Mi Hwang Jeong-Won Bae Dae-Jin Jung Jun Koo Yi Sang Moo Lee Jae Jung Ha Woo-Sung Kwon |
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Institution: | 1. Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Gyeongsangbuk-do, Republic of Korea ; 2. Gyeongbuk Livestock Research Institute, Yeongju Gyeongsangbuk-do, Republic of Korea ; 3. Department of Animal Biotechnology, Kyungpook National University, Sangju, Gyeongsangbuk-do, Republic of Korea |
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Abstract: | Although cryopreservation is an efficient method for maintaining the biological and genetic resources of sperm, the sperm damage during the cryopreservation process cannot be ignored. It should be possible to obtain the most effective cryopreservation performance by accurately grasping the effects of various factors on the cryopreservation of sperm. The previous study demonstrated that a suitable standard protocol for cryopreservation of Korean native brindled cattle (Chikso) does not exist, based on the methods for semen cryopreservation of Chikso differ in each research center. The most obvious difference between most of protocols is the addition of glycerol before and after cooling during the Chikso cryopreserved semen process. Therefore we focused on the effects of glycerol addition time on the quality of cryopreserved Chikso sperm. In the present study, 27 individual Chikso samples were collected by transrectal massage and divided into two parts: the “cryopreservation method A” group (adding glycerol before cooling) and the “cryopreservation method B” group (adding glycerol after cooling). Meanwhile, the values of various sperm parameters were derived from each group, including sperm motility, kinematics, capacitation status, cell viability, and intracellular ATP levels, which we used to compare and evaluate sperm function. The results of this study indicated that during the semen cryopreservation process of the Chikso, the addition of glycerol after cooling yielded superior results in a variety of sperm parameters, such as sperm motility, progressive motility, rapid motility, VCL, VSL, VAP, ALH, capacitation status, viability, and intracellular ATP level after freezing and thawing. Our study is suggested that the glycerol addition time during the cryopreservation process for Chikso should be considered. In addition, our results may be provided reference to develop suitable the cryopreservation procedure of the Chikso sperm. |
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Keywords: | cryopreservation glycerol adding time Korean native brindle cattle (Chikso) Sperm functions |
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