| 从构建的噬菌体抗体库中筛选抗甲肝人单抗 |
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| 引用本文： | 万泽生 王海涛 姜绍谆. 从构建的噬菌体抗体库中筛选抗甲肝人单抗[J]. 生物工程学报, 1998, 14(3): 294-297 |
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| 作者姓名： | 万泽生 王海涛 姜绍谆 |
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| 作者单位： | 1.第三军医大学微生物学教研室 重庆 400038；2.军事医学科学院微生物流行病研究所 北京 100071；3.第四军医大学微生物学教研室 西安 710032 |
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| 摘 要： | 用固相化甲肝抗原，对所构建的噬菌体抗体库进行了3轮淘筛。第3轮淘筛后洗脱下来的噬菌体，较第l轮增加了近100倍。含有抗体重链基因和轻链基因的重组克隆．也由淘筛前的25％增至100％。说明甲肝抗原对抗体库的淘筛，富集了表面呈现甲肝人单抗的噬菌体。经夹心ELISA法筛选到抗甲肝病毒噬菌体抗体，并以竞争抑制实验进一步证实了这些抗体的特异性。
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| 关 键 词： | 噬菌体抗体库，人单克隆抗体，甲肝病毒 |
Selection of Human Anti HAV McAb from a Phage Antibody Library |
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| Wan Zesheng Wang Haitao Jiang Shaozhun. Selection of Human Anti HAV McAb from a Phage Antibody Library[J]. Chinese journal of biotechnology, 1998, 14(3): 294-297 |
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| Authors: | Wan Zesheng Wang Haitao Jiang Shaozhun |
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| Affiliation: | Wan Zesheng 1 Wang Haitao 2 Jiang Shaozhun 3 |
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| Abstract: | The phage displaying antibody fragments were subjected to three rounds of panning with HAV antigen in solid phase.The eluted phage was enriched nearly 100 fold,and the percentage of recombinant clones increased from 25% to 100% after three rounds of panning.The HAV antigen specific monoclonal antibodies were selected by sandwich ELISA,and the specificity of these antibodies was further confirmed by competive inhibition ELISA. |
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| Keywords: | Phage antibody library human McAb hepatitis A virus |
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