| 维生素C发酵中2—酮—L—古龙酸还原酶的研究 |
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| 引用本文： | 蒋宇扬,郭振勇.维生素C发酵中2—酮—L—古龙酸还原酶的研究[J].生物工程学报,1998,14(3):339-341. |
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| 作者姓名： | 蒋宇扬 郭振勇 |
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| 作者单位： | 中科院沈阳应用生态研究所 |
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| 基金项目： | 国家“八、五”攻关项目 |
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| 摘 要： | 由于２酮Ｌ古龙酸还原酶（简称ＫＧＲ）的存在，理论上造成了Ｖｃ合成前体２酮Ｌ古龙酸（简称２ＫＬＧ）部分被还原成Ｌ艾杜糖酸［１，２］，影响了Ｖｃ二步发酵的产率。而从Ｌ山梨糖到２酮Ｌ古龙酸的转化中，除被ＫＧＲ催化的还原负反应外，均属...
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| 关 键 词： | 维生素C发酵 KGR 性质 活性 稀土元素化合物 |
Study on 2 keto L gulonate Reductase in Vitamin C Fermentation |
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| Jiang Yuyang\ Guo Zhenyong\ Jin Suying\ Lin Jishang\ Zhang Chenggang.Study on 2 keto L gulonate Reductase in Vitamin C Fermentation[J].Chinese Journal of Biotechnology,1998,14(3):339-341. |
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| Authors: | Jiang Yuyang\ Guo Zhenyong\ Jin Suying\ Lin Jishang\ Zhang Chenggang |
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| Abstract: | keto L gulonate reductase is composed of two Subunits,one with MW 32kDa,the other with 53kDa.Its isoelectric point is pI 3.6.The content of acidic amino acid is 22% of tatal protein.It is found that the rare eanth metal compounds have a surpressive effect of on the activity of 2 keto L gulonate reduetase.Using such rare earith metal compounds in the two steps fermentation of Vc,the fermentation time is shortened by 6 hours and the product of 2 keto L gulonic acid is increased by 10% more. |
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| Keywords: | keto L gulonate reductase depressant rare earth metal compound |
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