| 苯丙氨酸脱氨酶cDNA在大肠杆菌中的克隆与表达 |
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| 引用本文： | 刘敬忠 向华 胡维 朱章菱 祝瑾 李氢元. 苯丙氨酸脱氨酶cDNA在大肠杆菌中的克隆与表达[J]. 生物工程学报, 1998, 14(4): 384-388 |
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| 作者姓名： | 刘敬忠 向华 胡维 朱章菱 祝瑾 李氢元 |
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| 作者单位： | 首都医科大学附属北京红十字朝阳医院 北京 100020 |
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| 摘 要： |
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| 关 键 词： | 苯丙氨酸脱氨酶基因，基因表达，大肠杆菌，苯丙酮尿症 |
Cloning and Expression of Phenylalanine Ammonia Lyase cDNA in Escherichia coli |
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| Liu Jingzhong Xiang Hua Hu Wei Zhu Zhangling Zhu Jin Li Qingyuan. Cloning and Expression of Phenylalanine Ammonia Lyase cDNA in Escherichia coli[J]. Chinese journal of biotechnology, 1998, 14(4): 384-388 |
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| Authors: | Liu Jingzhong Xiang Hua Hu Wei Zhu Zhangling Zhu Jin Li Qingyuan |
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| Abstract: | The phenylalanine ammonia lyase (PAL) cDNA was amplified from Petroselinum crispum total RNA by using RT PCR technique.The amplified 2.2kb DNA fragment was inserted into expression vecter pET23b.The resulting plasmid pET23bPAL was sequenced and then transformed into E.coli JM109DE3.The expressed PAL protein in JM109DE3(pET23bPAL)accounted for more than 15% of the total proteins in the recombinent E.coli cell.The activity and specificity of the expressed PAL was identified and tested by using HPLC technique,and showed good enough for application to enzymatic therapy of PKU. |
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| Keywords: | Phenylalanine ammonia lyase gene expression E.coli PKU |
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