神经生长因子在6－OHDA化学性去交感神经中的保护作用

本实验用６－ＯＨＤＡ造成成年小白鼠领下腺化学性去交感神经，观察了神经生长因子对该神经的保护作用。６－ＯＨＤＡ（１５ｍｇ／ｋｇｉｐ）处理后２４ｈ腺体内去甲肾上腺素（ＮＥ）含量降至正常水平的２％以下。若在６－ＯＨＤＡ处理同时开始多次给予神经生长因子（ＮＧＦ），则ＮＥ残留量明显提高。减小６－ＯＨＤＡ剂量至１０ｍｇ／ｋｇ，ＮＥ残留量增加，同时ＮＧＦ的作用亦较用６－ＯＨＤＡ１５ｍｇ／ｋｇ时更为显著。若提前２４ｈ给予ＮＧＦ，尽管仍显著提高ＮＥ残留量，但程度却显然低于与６－ＯＨＤＡ同时给予者。以上结果表明外源性ＮＧＦ对６－ＯＨＤＡ造成交感神经化学性损毁有保护作用，此作用与神经受损的严重程度以及ＮＧＦ处理时间有关。

NERVE-PROTECTING EFFECTS OF NERVE GROWTH FACTOR(NGF)ON CHEMICAL SYMPATHECTOMY BY 6-OHDA

Chemical sympathectomy of submandibular glands in adult mice by 6-OHDA was per-formed and the nerve-protecting effect of nerve growth factor(NGF)was investigated.24 hoursafter 6-OHDA(15mg/kg ip) lesion,NE content in the glands decreased to less than 2% of thenormal level and was greatly raised when NGF(multidose)treatment began at the same time of6-OHDA lesion. After injection of lower dosage of 6-OHDA(10mg/kg),the NE level washigher and the effect of NGF was more significant than those in 6-OHDA 15mg/kg group. However,when the treatment of NGF began 24 hours preceding the 6-OHDA(10mg/kg)lesion,it remained effective but in a much less degree. These results suggest that exogenous NGFis effective in protecting the sympathetic nerve from the chemical lesion of 6-OHDA,and the ef-fect is influenced by the severity of the lesion and the time of NGF administration.