Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells |
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Authors: | Cotter Gary Weedle Roisin Kavanagh Kevin |
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Institution: | (1) Medical Mycology Unit, Department of Biology, National University of Ireland, Maynooth, Co, Kildare, Ireland |
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Abstract: | The presence of the extracellular matrix (ECM) proteins collagen types I and IV, laminin and fibronectin on the surface of
HEp-2 cells was confirmed by flow cytometry using monoclonal antibodies. Monoclonal antibodies directed against these ECM
proteins reduced the adherence of C. albicans ATCC 44990 to HEp-2 cells, the greatest reductions being evident in assays which
incorporated anti-collagen type IV monoclonal antibody. The ability of sugaramines to inhibit the adherence of C. albicans
to a variety of cell types has been demonstrated previously and the most significant reduction in C. albicans – HEp-2 adherence
was in assays which incorporated 0.2M galactosamine. The combination of anti-collagen IV monoclonal antibody and galactosamine
reduced the adherence of C. albicans to HEp-2 cells by approximately 70% (p < 0.05).
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Adherence Candida Extracellular matrix HEp-2 |
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