Effects of an Agropyron chromosome on endosperm proteins in common wheat (Triticum aestivum L.) |
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Authors: | K. M. Soliman J. E. Bernardin C. O. Qualset |
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Affiliation: | (1) Department of Agronomy and Range Science, University of California, 95616 Davis, California;(2) U.S. Department of Agriculture, Western Regional Research Laboratory, 94710 Berkeley, California |
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Abstract: | An Agropyron chromosome having a gene conferring blue color on the aleurone layer of the kernel endosperm causes a 15% increase in total grain protein content when it is added to the common wheat (2n=42) complement. In contrast, there is no effect of this chromosome on total protein content if it replaced part of a wheat chromosome. Endosperm protein components of isolines having blue aleurone due to the Agropyron chromosome being added (2n=44) or translocated (2n=42) were compared to normal nonblue isoline counterparts. Gliadin proteins separated by aluminum lactate (pH 3.2) polyacrylamide gel electrophoresis (PAGE) in one or two dimensions showed greater staining intensity for the blue addition isolines (2n=44) than nonblue (2n=42) isolines. However, the 42-chromosome blue isoline did not show increased protein staining over the nonblue isoline, but at least five protein differences were detected between the lines. SDS-PAGE showed that blue and nonblue differences were expressed primarily in the gliadins, but also in the glutenin, globulin, and albumin proteins.This research was supported by a D. F. Jones Postdoctoral Fellowship to K. M. Soliman and by Western Regional Project W-132, Genotype-environment interactions related to end-product uses in small grains. |
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Keywords: | Agropyron elongatum endosperm protein gliadins glutenin chromosome addition lines isolines gel electrophoresis SDS-PAGE |
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