Monocyte-chemoattractant-protein-1-mediated migration of human monocytes towards blasts from patients with acute myeloid leukemia |
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Authors: | Maria C J C Legdeur Marjolein G Broekhoven Gerrit J Schuurhuis Robert H J Beelen Gert J Ossenkoppele |
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Institution: | (1) Department of Hematology, BR 240, University Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands e-mail: g.ossenkoppele@azvu.nl Tel.: +31-20-4442604; Fax: 31-20-4442601, NL;(2) Department of Cell Biology, University Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL |
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Abstract: | Purpose: In the present study the possible clinical relevance of monocyte chemoattractant protein (MCP)-1 in patients with acute myeloid
leukemia (AML) was established. Methods: The pattern of migration of human monocytes towards the supernatants of blasts from 15 patients with AML was studied and
the role of MCP-1, produced by these blasts, was assessed. Results: In 4 patients (group 1) the amount of monocyte migration was low and not inhibited by the addition of anti-hMCP-1. In 11
patients, the amount of monocyte migration was high; after addition of anti-hMCP-1, monocyte migration was either completely
(8 patients, group 2), or partly or not (3 patients, group 3) inhibited to the level of chemokinesis. In groups 1 and 2, there
was a good correlation (r=0.67) between the concentration of MCP-1 in the supernatants and the amount of monocyte migration. In group 3, such a correlation
was not evident, suggesting that another chemokine might be involved or MCP-1 function was impaired by an unknown substance.
Finally, measurements of MCP-1 during culture of AML blasts showed that the time at which maximal amounts of MCP-1 are produced
differs between the AML samples. Conclusions: AML blasts produce different amounts of MCP-1, which plays an important role in monocyte migration towards most AML blasts.
Therefore, in the context of adoptive immunotherapy, MCP-1 might be involved in future tumor vaccination programmes using
autologous MCP-1-transfected irradiated AML blasts.
Received: 4 May 2000 / Accepted: 26 October 2000 |
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Keywords: | AML MCP-1 Monocyte migration |
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