Cloning and characterization of the Rubisco activase gene from <Emphasis Type="Italic">Ipomoea batatas</Emphasis> (L.) Lam |
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Authors: | Ke Xu Bowen He Shuang Zhou Yi Li Yizheng Zhang |
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Institution: | (1) Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Science, Sichuan University, 610064 Chengdu, People’s Republic of China |
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Abstract: | A full-length cDNA of Rubisco activase (IBrcaI) was cloned from sweet potato (Ipomoea batatas (L.) Lam) using Rapid-Amplification of cDNA Ends (RACE). IBrcaI contains a 1,347 bp open reading frame encoding a protein of 439 amino acids. The sequence alignment of multiple Rubisco
activase genes from sweet potato and other plants showed high homology at two previously described ATP-binding sites. Western
blot analysis indicated that there are two Rubisco activase proteins in sweet potato. Expression of IBrcaI was only detected in leaves. In the 14 h light and 10 h dark photoperiods, maximal and minimal IBrcaI mRNA expression levels were detected at 8:00 in the morning and at midnight, respectively. |
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