Response surface optimization of polysaccharides extraction from liriope roots and its modulatory effect on sjogren syndrome |
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| Authors: | Li Mei Wang Zhen-Chang Dai Hao-Jie Cui Li Xu Qing-Gang Li Jing |
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| Affiliation: | aDepartment of Nuclear Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;bDepartment of Radiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;cDepartment of Rheumatism, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China |
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| Abstract: | The response surface methodology (RSM) was employed to study the extraction of liriope polysaccharides. The maximum extraction rate (64.1%) of liriope polysaccharides obtained by using the above optimised concentrations of the variables was extraction time (X1) at 185 min, ratio of liquid to solid (X2) at 11, and extraction temperature (X3) at 94 °C. Forty male Wistar rats were used in the present experiment. After 2 weeks of acclimatization, animals were divided into 5 equal groups: control, SS model and 3 polysaccharides-treatment groups. Rats were orally administered their respective doses every day for 6 weeks. Liriope polysaccharides significantly increased the amount of salivary secretion, and the relative weight of spleen, thymus and submandibular glands. Therefore, the present results revealed that liriope polysaccharides exert a protective effect against tissue damage in rats with sjogren syndrome (SS). |
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| Keywords: | Rat Liriope polysaccharides Extraction SS Response surface methodology (RSM) Optimization |
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