十二烷基硫酸钠对高压静电场对酶作用的影响

研究了十二烷基硫酸钠(SDS)对高压静电场对酶作用的影响。结果表明:在强度为3×103V/cm的电场作用下,十二烷基硫酸钠使过氧化氢酶对高压静电场的作用变得更加敏感。低浓度的十二烷基硫酸钠中,过氧化氢酶在高压静电场的作用下活力升高;0.04mmol/L～0.12mmol/L的十二烷基硫酸钠中,酶活力在高压静电场作用下先升高后下降;高于0.40mmol/L,则酶活力单调下降。十二烷基硫酸钠对高压静电场中的过氧化氢酶具有一定的保护作用。

The Effects of SDS on Interaction of High Voltage Electrostatic Field on Catalase

The effect of SDS on inte raction of high voltage electrostatic field(HVEF) of catalase was studied. The r esults indicated that: SDS made that catalase was more sensitive in high voltage electrostatic field than C. K. At 3×10~3v／cm.　when　the　co ncentration　of　SDS　was　0.02mmol／L,　the　a ctivity　of　catalase　increased　greatly　in high　voltage　electrostatic　field.　If　th e　concentration　of　SDS　was　between　0.04m mol／L　and　0.12mmol／L,　the　activity　of　ca talase　increased　firstly　and　then　decrea sed.　When　the　concentration　was　between 0.20mmol／L　and　0.60mmol／L,　the　activity of　catalase　in　high　voltage　electrostati c　field　decreased　lonely.　SDS　could　some what　protect　catalase　from　high　voltage electrostatic.　The　mechanism　was　probabl y that　SDS　could　form　an　opposite　electro static　field　when　HVEF　is　on　solution　of SDS　and　catalase,　and　the　electronic　fi eld　strength　reduced.