Glucose uptake and phosphorylating activities in two species of slow-growing Rhizobium

Abstract Glucose uptake and phosphorylating activities were studied in two strains of slow-growing Rhizobium: Rhizobium japonicum (USDAI-110) and cowpea Rhizobium (USDA3278). Cultured cells of both species actively took up glucose, and at least two systems appeared to be involved, whereas purified bacteroids of both species failed to accumulate glucose actively. In both cell types, glucose phosphorylation was ATP-dependent, and no evidence was obtained for a phosphoenolpyruvate-dependent glucose phosphotransferase system.