Constitutive over-expression of two wheat pathogenesis-related genes enhances resistance of tobacco plants to Phytophthora nicotianae |
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| Authors: | Floriana Fiocchetti Rosalinda D’Amore Monica De Palma Laura Bertini Carla Caruso Carlo Caporale Antonino Testa Gennaro Cristinzio Francesco Saccardo Marina Tucci |
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| Institution: | (1) Department of Plant Production, University of Tuscia, Via S. Camillo De Lellis, Viterbo, 01100, Italy;(2) Present address: ENEA C.R. Casaccia, Department of Biotechnologies, Agroindustry and Health Protection, Via Anguillarese 301, Rome, 00123, Italy;(3) CNR – Institute of Plant Genetics, Via Università 133, Portici, 80055, Italy;(4) Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo De Lellis, Viterbo, 01100, Italy;(5) Department of Ar.Bo.Pa.Ve. - Plant Pathology, University of Naples, Via Università 100, Portici, 80055, Italy |
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| Abstract: | The potential role in plant defence of the two wheat pathogenesis-related proteins of class 4 Wheatwin1 and Wheatwin2, possessing
high in vitro antimicrobial activity against several pathogens, was investigated through over-expression of their encoding
genes wPR4a and wPR4b in transgenic tobacco plants. Several independent transformants were obtained, expressing high levels of either transgene
when analysed by northern and western blotting. Accumulation of the wPR4b-encoded protein Wheatwin2 in the apoplast of transgenic plants was also demonstrated. When homozygous transgenic lines in
the T4 generation were tested for increased tolerance to Phytophthora nicotianae, they were found to be significantly more resistant than both the wild type and their isogenic, non-wPR4 transgenic lines. These results suggest that both Wheatwins might have in vivo antimicrobial activity, confirming earlier
indications from in vitro assays. |
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| Keywords: | Antimicrobial activity Antimicrobial proteins Plant defence PR4 genes Disease resistance |
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