The possibility of aromatization of androgen in human prostate |
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Authors: | Y Kaburagi M B Marino R Y Kirdani J P Greco J P Karr A A Sandberg |
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Affiliation: | 1. Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada;2. Department of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada;3. Estación de Biología Chamela, Instituto de Biología, Universidad Nacional Autónoma de México, San Patricio, Jalisco, Mexico;4. Department of Psychology, Neuroscience & Behaviour, McMaster University, Hamilton, Ontario, Canada |
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Abstract: | Aromatase in human prostate tissue was determined in homogenized human prostate (three BPH and two normal specimens) incubated with [1-beta-3H]androstenedione (radiometric method) or [1,2,6,7-3H]androstenedione (estrogen production analysis method) in the presence of NADPH. Using the former procedure, significant amounts of 3H2O, resulting from the release of 3H at the C-1 position during aromatization, were measured and these increased with incubation time and amount of tissue, whereas the amount of estrone and estradiol-17 beta resulting from the latter method and calculated from the 3H/14C ratio in preparations of purified crystal was very small. The preliminary results, which suggest that an androgen aromatase system exists in the human prostate, point to the need to further investigate the identity and properties of the metabolic products resulting from the conversion of androgen to estrogens and other metabolites. |
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