Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6-keto F1alpha and thromboxane B2 on cyclic AMP production and adenylate cyclase activity. |
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| Authors: | A Crawford D Atkins T J Martin |
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| Affiliation: | 1. Department of Chemical Pathology, University of Sheffield, Medical School, Sheffield, S10 2RX, U.K.;2. Department of Medicine, University of Melbourne, Repatriation General Hospital, Heidelberg, Victoria, Australia. |
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| Abstract: | The effects of prostaglandins (PG's) E1, E2, I2 (prostacyclin), 6-keto F1α and thromboxane (Tx) B2 were compared in freshly isolated cells from a rat osteogenic sarcoma and in membranes from cultured cells of the same tumour. Cyclic AMP production was measured in cells and adenylate cyclase activity was measured in cell membranes. In both systems PGI2 was less potent than either of the PGE's, and both TxB2 and 6-keto PGF1α were only weak agonists. These effects on bone-derived cells suggest that PGI2 is unlikely to be a potent bone resorbing agent. Resitance experiments suggested that all the PG's share the same receptor site which appears distinct from the site of action of parathyroid hormone. |
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| Keywords: | PG prostaglandin Tx thromboxane PTH parathyroid hormone cyclic AMP adenosine-3′,5′-cyclic monophosphate PBS phosphate buffered saline EDTA ethylenediaminetetra-acetic acid ATP adenosine triphosphate MEM minimal essential Eagle's medium |
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