A new method of optical detection of yeast acidification power |
| |
Authors: | P Gabriel M Dienstbier P Sladký K Sigler |
| |
Institution: | (1) Faculty of Mathematics and Physics, Charles University, 121 16 Prague, Czech Republic;(2) Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic |
| |
Abstract: | We describe here a newly developed method for a contact-free optical pH measurement in yeast suspensions supplemented with
glucose, and containing the pH sensitive triphenylmethane dye bromocresol green. It is suitable for performing the acidification
power test (based on measuring the rate of pH drop of yeast suspension caused by active extrusion of acidity from cells after
glucose addition) used for assessing yeast vitality in fermentation industries. Using this methodology we monitored the pH
in yeast suspensions in the course of acidification in the pH range of 3.5–5.3. Optical pH measurement allows simultaneous
testing of several samples, minimizes the sample volume, simplifies sample handling and reduces the hands-on time in sample
processing. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|