Evaluation of cytochrome b mtDNA sequences in genetic diversity studies of Channa marulius (Channidae: Perciformes) |
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Authors: | Maria Habib W. S. Lakra Vindhya Mohindra Praveen Khare A. S. Barman Akanksha Singh Kuldeep K. Lal Peyush Punia Asif A. Khan |
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Affiliation: | (1) National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, UP, 226002, India;(2) Department of Zoology, Faculty of Life Science, Aligarh Muslim University, Aligarh, UP, 202002, India; |
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Abstract: | Channa marulius (Hamilton, 1822) is a commercially important freshwater fish and a potential candidate species for aquaculture. The present study evaluated partial Cytochrome b gene sequence of mtDNA for determining the genetic variation in wild populations of C. marulius. Genomic DNA extracted from C. marulius samples (n = 23) belonging to 3 distant rivers; Mahanadi, Teesta and Yamuna was analyzed. Sequencing of 307 bp Cytochrome b mtDNA fragment revealed the presence of 5 haplotypes with haplotype diversity value of 0.763 and nucleotide diversity value of 0.0128. Single population specific haplotype was observed in Mahanadi and Yamuna samples and 3 haplotypes in Teesta samples. The analysis of data demonstrated the suitability of partial Cytochrome b sequence in determining the genetic diversity in C. marulius population. |
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