Genetic variation and phylogenetic relationship between two species of yellow catfish, <Emphasis Type="Italic">Horabagrus brachysoma</Emphasis> and <Emphasis Type="Italic">H. nigricollaris</Emphasis> (Teleostei: Horabagridae) based on RAPD and microsatellite markers |
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Authors: | P M Abdul Muneer A Gopalakrishnan Remya Shivanandan V S Basheer A G Ponniah |
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Institution: | (1) National Bureau of Fish Genetic Resources (NBFGR) Cochin Unit, CMFRI Campus, Cochin, 682 018, Kerala, India;(2) Central Institute of Brackish water Aquaculture (CIBA), 75 Santhome High Road, Near MRC Nagar, R.A. Puram, Chennai, 600 028, Tamil Nadu, India;(3) Neurovascular Oxidative Injury Laboratory, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198-5215, USA |
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Abstract: | The two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris are categorized as ‘endangered’ and ‘critically endangered’ respectively in their wild habitat. Proper knowledge of genetic
structure and variability of these endangered species are highly essential for the management, conservation and improvement
of fish stocks. Therefore, genetic variation and phylogenetic relationships between these species of yellow catfish sampled
from Chalakkudy River in the hot spot of biodiversity-Western Ghats region, Kerala, India were analyzed by using Random amplified
polymorphic DNA (RAPD) and microsatellite markers. 85 RAPD and five microsatellites loci were detected to analyze the genetic
variation and phylogenetic relationships among these species. Out of 85 RAPD loci produced only 52.94% were polymorphic whereas
in microsatellite, all 5 loci were polymorphic (100%). Species-specific RAPD bands were found in both species studied. In
microsatellite, the number of alleles across the five loci ranged from 1 to 8. The observed heterozygosities in H. brachysoma and H. nigricollaris were 0.463 and 0.443, respectively. Here, both RAPD and microsatellite methods reported a low degree of gene diversity and
lack of genetic heterogeneity in both species of Horabagrus which strongly emphasize the need of fishery management, conservation and rehabilitation of these species. |
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