Identification of mouse MARVELD1 as a microtubule associated protein that inhibits cell cycle progression and migration |
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Authors: | Fanli Zeng Yanyan Tian Shuliang Shi Qiong Wu Shanshan Liu Hongxia Zheng Lei Yue Yu Li |
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Institution: | Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, 150001, People's Republic of China. |
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Abstract: | MARVEL domain-containing 1 (MARVELD1) is a newly identified nuclear protein; however its function has not been clear until
now. Here, we report that mouse MARVELD1 (mMARVELD1), which is highly conserved between mice and humans, exhibits cell cycle-dependent
cellular localization. In NIH3T3 cells, MARVELD1 was observed in the nucleus and at the perinuclear region during interphase,
but was localized at the mitotic spindle and midbody at metaphase, and a significant fraction of mMARVELD1 translocated to
the plasma membrane during anaphase. In addition, treatment of cells with colchicine, a microtubuledepolymerizing agent, resulted
in translocation of mMARVELD1 to the plasma membrane, and association of mMARVELD1 and α-tubulin was confirmed by co-immunoprecipitation.
Finally, overexpression of mMARVELD1 resulted in a remarkable inhibition of cell proliferation, G1-phase arrest, and reduced
cell migration. These findings indicate that mMARVELD1 is a microtubule-associated protein that plays an important role in
cell cycle progression and migration. |
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Keywords: | cell migration cell-cycle progression G1-phase arrest MARVELD1 microtubule-associated protein |
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