Pigment and isozyme variation in aspen shoots regenerated from callus culture |
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Authors: | Eun-Woon Noh Subhash C Minocha |
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Institution: | (1) Department of Plant Biology, University of New Hampshire, 03824 Durham, NH, USA |
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Abstract: | Pigment as well as isozyme variations were observed among aspen (Populus tremuloides Michx.) plants regenerated from callus cultures. Out of more than 600 plantlets, two chimeric plants (one with green base and two albino shoots and the other with an albino shoot) were produced. Callus derived from albino shoots produced albino as well as chimeric plants when transferred to shoot inducing medium. Isozyme patterns of 119 plants were examined by starch gel electrophoresis. Thirty plants showed variation in shikimic dehydrogenase isozyme and 41 in isocitric dehydrogenase. Variation was also observed in malate dehydrogenase and phosphoglucose isomerase. No variation was seen in 6-phosphogluconate dehydrogenase. Pigment variation was not associated with any isozyme changes.Abbreviations BA
6-benzyladenine
- IBA
indole-3-butyric acid
- GD
Gresshoff & Doy medium
- WPM
woody plant medium
- SKD
shikimic dehydrogenase
- IDH
isocitric dehydrogenase
- MDH
malate dehydrogenase
- PGI
phosphoglucose isomerase
- 6-PGD
6-phosphogluconate dehydrogenase |
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Keywords: | albino aspen chimera callus culture somaclonal variation |
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