Expression of nitrile hydratase gene of mutant 4D strain of Rhodococcus rhodochrous PA 34 in Pichia pastoris |
| |
| Authors: | Amit Pratush Amit Seth |
| |
| Institution: | 1. Department of Bioengineering, School of life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China,;2. Department of Bioengineering, Shoolini University of Biotechnology and management Sciences, Solan, India, and |
| |
| Abstract: | The nitrile hydratase (NHase) gene of Rhodococcus rhodochrous PA-34 mutant 4D has been amplified by PCR, cloned and expressed in Pichia pastoris KM-71 using pHIL-D2 expression vector. The recombinant P. pastoris KM-71 exhibited active expression of the nitrile hydratase gene of the mutant 4D and has shown very good potential for the transformation of 3-cyanopyridine to nicotinamide. The recombinant P. pastoris KM-71 exhibited maximum NHase activity when cultivated in YPD medium was supplemented with 0.4?mM cobalt ions. The recombinant P. pastoris KM-71 showed maximum nitrile hydratase enzyme production, when incubated at 30?°C for 15?h. |
| |
| Keywords: | Mutant nitrile hydratase recombinant P pastoris Rhodococcus rhodochrous PA-34 |
|
|
