AFLP markers for the study of rice biodiversity |
| |
Authors: | J Zhu M D Gale S Quarrie M T Jackson G J Bryan |
| |
Institution: | (1) John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK Fax: +44-01603 502241 E-mail: zhu@bbsrc.ac.uk, GB;(2) International Rice Research Institute, PO Box 933, 1099 Manila, Philippines, XX |
| |
Abstract: | AFLP was used as a DNA fingerprinting technique in rice (Oryza sativa L.) germplasm analysis. The high efficiency and random coverage of AFLP markers were established. With only five combinations
of primers and RFLP anchors, a framework linkage map was constructed. This map demonstrated that the AFLP markers from a limited
number of primers were not confined to any particular regions or chromosomes in the rice genome. To analyse the biodiversity
of 57 rice germplasm accessions, we examined 179 polymorphic AFLP markers generated from four primer combinations. Both principal
component analysis and cluster analysis were used, and three groups were clearly identified which corresponded to genotypes
of Isozyme Groups I, II and VI. The number of markers needed for robust classification of rice germplasm and the diversity
between/within the groups was established.
Received: 15 July 1997 / Accepted: 29 October 1997 |
| |
Keywords: | AFLP Rice Biodiversity Germplasm Mapping |
本文献已被 SpringerLink 等数据库收录! |
|