Bacteriocin production by Enterococcus faecium NA01 from 'wara'—a fermented skimmed cow milk product from West Africa |
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Authors: | NA Olasupo U Schillinger CMAP Franz WH Holzapfel |
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Institution: | Department of Botany and Microbiology, Lagos State University Ojo, Apapa, Lagos, Nigeria;Federal Research Centre for Nutrition, Institute of Hygiene and Toxicology, Karlsruhe, Germany |
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Abstract: | An Enterococcus faecium strain from Nigerian fermented skimmed cow milk ('wara') produced bacteriocin inhibitory towards Lactobacillus, Enterococcus and Listeria strains. The bacteriocin (designated enterocin 01) was inactivated by proteases, heat-stable at 100°C and active at pH 2.0–6.0. The Ent. faecium isolate harboured plasmids of ca 36.3 and 23.1 kb. Curing experiments with ethidium bromide resulted in a bacteriocin-negative mutant which had not lost immunity to the bacteriocin. Slight differences in plasmid profiles between wild-type and mutant indicated a possible plasmid-coded bacteriocin production. |
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