Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, <Emphasis Type="Italic">Periplaneta americana</Emphasis> as inferred from co-localization of Rab8, PTTH,and protein kinase C in neurosecretory cells |
| |
Authors: | Susumu Hiragaki Tomohide Uno Makio Takeda |
| |
Institution: | (1) Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657 8501, Japan;(2) Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku Hyogo, Kobe 657 8501, Japan |
| |
Abstract: | Small GTPases of the Rab family act as essential regulators of vesicle transport pathways, including the exocytosis of neurohormones.
These processes are not well-understood in insects. To address the physiological function of Rab proteins and their phosphorylation
in insect neurosecretion, Rab8-like, prothoracicotropic hormone (PTTH)-like, and protein kinase C (PKC)-like immunohistochemical
reactivities (-ir) were investigated in the brain of the American cockroach, Periplaneta americana. All the antibodies tested reacted with neurons in the pars intercerebralis, corpora cardiaca, and nervi corporis allati
I. Double-labeling experiments demonstrated that all PTTH-ir were colocalized with Rab8-ir and PKC-ir in the pars intercerebralis,
although exclusive reactivity was present to antisera against Rab8 or PKC. These findings support the notion that Rab8-like
antigen is phosphorylated by PKC, and that this phosphorylation is involved in the axonal transport and secretion of PTTH
in this species.
This work was supported by a Grant-in-Aid for Scientific Research (B) 1838043, (C) 20580053, and Global Center of Education
and Research Program (19GCOE01) from the Japan Society for the Promotion of Science. |
| |
Keywords: | Rab protein Neurosecretion Protein kinase C Prothoracicotropic hormone Periplaneta americana (Insecta) |
本文献已被 SpringerLink 等数据库收录! |
|