Structural characterization of the O-polysaccharide antigen of Edwardsiella tarda MT 108 |
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Authors: | Vinogradov Evgeny Nossova Ludmila Perry Malcolm B Kay William W |
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Affiliation: | Institute for Biological Sciences, National Research Council, Ottawa, ON, K1A OR6, Canada. |
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Abstract: | Edwardsiella tarda, a Gram-negative bacterium, is an important cause of hemorrhagic septicemia in fish and also of gastro- and extraintestinal infections in humans. The lipopolysaccharide produced by the fish pathogenic strain E. tarda MT 108 was isolated and the structure of its antigenic O-polysaccharide component determined by the application of chemical analyses, high-resolution 1D and 2D nuclear magnetic resonance spectroscopy, and mass spectrometry. The polysaccharide was found to be a polymer of a repeating pentasaccharide unit composed of 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 2-acetamido-2-deoxy-D-galactose (D-GalNAc), D-galactose (D-Gal), L-rhamnose (L-Rha), D-galacturonic acid (D-GalA) and (2S,3R)-threonine (1:1:1:1:1:1) having the structure: [structure: see text]. |
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Keywords: | Lipopolysaccharide Edwardsiella tarda, O-antigen |
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