Production and characterization of somatic hybrids between <Emphasis Type="Italic">Solanum tuberosum</Emphasis> L. and <Emphasis Type="Italic">S. pinnatisectum</Emphasis> Dun. |
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Authors: | D Sarkar Jagesh K Tiwari Sushruti Sharma Poonam Sanjeev Sharma J Gopal B P Singh S K Luthra S K Pandey D Pattanayak |
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Institution: | (1) Central Potato Research Institute (CPRI), Shimla, Himachal Pradesh, 171 001, India;(2) Central Potato Research Institute Campus, Modipuram, Meerut, Uttar Pradesh, 250 110, India;(3) Present address: Division of Crop Improvement, Central Research Institute for Jute and Allied Fibres (CRIJAF), Barrackpore, Kolkata, West Bengal, 700 120, India; |
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Abstract: | Interspecific somatic hybrids between the dihaploid Solanum tuberosum and the wild species S. pinnatisectum Dun. were produced via protoplast fusion. Protoplast isolation, electrofusion, culture of post-fusion products and regeneration
of calli/shoots were undertaken following optimized protocols. Regenerants were characterized for hybridity, ploidy and resistance
to Phytophthora infestans (Mont.) de Bery, causal fungal pathogen of late blight disease. From a total of 126 regenerated macrocalli, 12 somatic hybrids
were confirmed by possessing species-specific diagnostic bands of their corresponding parents as revealed by RAPD, SSRs and
cytoplasmic-DNA analyses. Tetraploid status of the 12 hybrids was determined using flow cytometry analysis. Intermediate phenotypes
for leaf, flower, and tuber characteristics and high male fertility were observed in field-grown hybrid plants. Hybrids were
highly resistant to foliage late blight based on field assessment for two seasons. In contrast, moderate level of resistance
to foliage blight was observed in hybrids based on the detached leaf assay under laboratory conditions. Overall, somatic hybrids
with moderate levels of resistance to foliage blight were identified, and these will be useful for in situ hybridization in
potato breeding efforts. |
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