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Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes |
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| Authors: | Kameoka Masanori Kitagawa Yukiko Utachee Piraporn Jinnopat Piyamat Dhepakson Panadda Isarangkura-na-ayuthaya Panasda Tokunaga Kenzo Sato Hironori Komano Jun Yamamoto Naoki Oguchi Shinobu Natori Yukikazu Ikuta Kazuyoshi |
| Institution: | Section of Viral infections, Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi 11000, Thailand. mkameoka@biken.osaka-u.ac.jp |
| Abstract: | We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication. |
| Keywords: | Human immunodeficiency virus type-1 RNA interference Small interfering RNA siRNA mini-library Host factor |
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