两种纤毛虫营养细胞和休眠细胞蛋白组成的比较分析

应用生化抽提和SDS-PAGE方法显示,膜状急纤虫(Tachysoma pellionella)的营养细胞含38条蛋白谱带,休眠细胞含29条蛋白谱带,两者共有谱带为26条,特有谱带各为12条和3条,相似度为77.6%;休眠细胞的包囊壁含22条蛋白谱带,细胞脱包囊后残留的包囊壁含15条蛋白谱带,两者共有谱带为14条,特有谱带各为8条和1条,相似度为76%。包囊游仆虫(Euplotes encysticus)的营养细胞和休眠细胞均含23条蛋白谱带,两者共有谱带为19条,特有谱带各为4条,相似度为82.6%;休眠细胞的包囊壁和细胞脱包囊后残留的包囊壁均含20条蛋白谱带,两者共有谱带为19条,特有谱带均为1条,相似度为95%。结果表明,两种纤毛虫的营养细胞向休眠细胞转化过程中,细胞结构的主要蛋白质成分发生了明显变化,这些变化与细胞在不同生理状态下结构的分化及其生命活动特征相关。形成“毛基体吸收型包囊”的急纤虫与形成“毛基体非吸收型包囊”的游仆虫相比较,前者营养期和休眠期细胞蛋白组成有更明显的差异,这可能与其细胞结构更大程度的脱分化有关;根据纤毛虫休眠细胞的包囊壁和细胞脱包囊后残留的包囊壁两者蛋白组成的差异推测,前者包囊壁可能含有与休眠细胞生命活动相联系的“活性”成分。

Comparative Analysis on the Protein Compositions Between Vegetative and Resting Cell of Two Ciliates

The protein compositions between vegetative cell and resting cell(cyst) of two fresh water ciliates are comparatively analyzed using biochemistry extraction and SDS-PAGE technique.The results show that the vegetative and resting cell of Tachysoma pellionella(TP) contains 38 and 29 protein bands,respectively.The former and the latter contain 12 and 3 specific protein bands,respectively.Both have 26 common protein bands.The similarity values is 77.6%.The cyst wall of resting cell of TP contains 22 protein bands.The remaining cyst wall contains 15 protein bands after TP excystment.The former and the latter contain 8 and 1 specific protein bands,respectively.Both have 14 common protein bands.The similarity values is 76%.The results also indicate that the vegetative and resting cell of Euplotes encysticus(EE) all contain 23 protein bands.Both have 19 common protein bands and all contain 4 specific protein bands.Their similarity values is 82.6%.The cyst wall of resting cell of EE and the remaining cyst wall after EE excystment all contain 20 protein bands.Both have 19 common protein bands and all contain 1 specific protein bands.Their similarity values is 95%.These data reveal that there are obviously variances of main protein components of two ciliates in the differentiating process of converting vegetative cell into resting cell.These variances of proteins are associated with structural differentiation of their cells and features of their life activity under different physiological condition.The protein composition in vegetative and resting cell of TP forming "kinetosome-resorbing cyst " has more variance as compared with that of EE forming "non-kinetosome-resorbing cyst",which may be related with structural dedifferetiation of TP in greater degree.From protein composition variance between the cell wall of resting cell of the ciliates and the remaining cell wall after the ciliates excystment,we infer that cell wall of resting cell of the ciliates possibly contains "active" components associated with life activity of the resting cell.