Adventitious bud induction in Eucalyptus globulus Labill |
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| Authors: | Greg Nugent Stephen F Chandler Phil Whiteman Trevor W Stevenson |
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| Institution: | (1) Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, GPO Box 2476V, 3001 Melbourne, Australia;(2) Florigene Pty. Ltd, 16 Gipps Street, 3066 Collingwood, Australia;(3) Australian Paper Plantations Pty. Ltd, P.O. Box 220, 3840 Morwell, Australia;(4) Present address: Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland |
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| Abstract: | Summary Adventitious buds and shoots of Eucalyptus globulus Labill. (Tasmanian Bluegum) have been regenerated from cotyledons and hypocotyls from mature embryos and seedlings. Adventitious
buds, were induced at high frequency with 0.05 μM thidiazuron in combination with 0.2 μM 2,4-dichlorophenoxyacetic acid or 5 μM α-naphthaleneacetic acid. Culture of explants in the dark inhibited bud induction, but up to 86% of cotyledons, longitudinally
split just prior to culture, produced adventitious buds, in the light. Development of buds into shoots occurred only at low
frequency, after transfer to media containing N6-benzylaminopurine. |
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| Keywords: | mature embryo organogenesis thidiazuron |
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