Multiple forms of xylose reductase in Pachysolen tannophilus CBS4044 |
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Authors: | Cornelis Verduyn Johannes Frank Jzn Johannes P. van Dijken W.Alexander Scheffers |
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Affiliation: | Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67A, 2628 BC Delft, The Netherlands |
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Abstract: | Abstract Cell-free extracts of xylose-grown Pachysolen tannophilus exhibited xylose reductase activity with both NADPH and NADH. The ratio of the NADPH- and NADH-dependent activities varied with growth conditions. Affinity chromatography of cell-free extracts resulted in a separation of two xylose reductases. One was active with both NADPH and NADH, the other was specific for NADPH. Apart from this coenzyme specificity, the two enzymes also differed in their affinities for xylose and NADPH. The role of the two enzymes in xylose metabolism is discussed in relation to attempts to use P. tannophilus for the alcoholic fermentation of wood sugars. |
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Keywords: | Xylose reductase xylose fermentation yeast wood sugar ethanol |
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