a Department of Endocrinology, Christchurch Hospital, Christchurch, New Zealand
b Department of Nuclear Medicine, Christchurch Hospital, Christchurch, New Zealand
Abstract:
A bivariate assay has been developed to measure both ANF, and cleaved ANF (ANF(101–105/106–126)) the primary product of endopeptidase-24.11 action on rat ANF(101–126). The assay employed two different antisera directed to the carboxy-terminal and ring regions of ANF to enable discrimination and measurement of intact endogenous ANF(99–126) and infused cleaved ANF (rat ANF(101–105/106–126)) in sheep plasma extracts. The assay which was validated by HPLC analysis of plasma extracts, had sufficient accuracy and precision to measure the metabolic clearance rate and half-life of cleaved ANF when infused in normal sheep.