Removal of lipopolysaccharide from acellular Bordetella pertussis vaccine by detergent treatment |
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| Authors: | R S Stinson R D Lemmon D W Baggett T L Huff J A Malone G L Sloan A L Winters |
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| Institution: | 1. Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea;2. International Vaccine Institute, Seoul, Republic of Korea;3. Cambridge Institute of Therapeutic Immunology and Infectious Disease, University of Cambridge School of Clinical Medicine, Cambridge Biomedical Campus, Cambridge, United Kingdom;4. Heidelberg Institute of Global Health, University of Heidelberg, Heidelberg, Germany;5. Madagascar Institute for Vaccine Research, University of Antananarivo, Antananarivo, Madagascar |
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| Abstract: | Protective antigen was extracted from Bordetella pertussis cells with 1.0 M NaCl and precipitated with ammonium sulfate, 20-40% saturation (designated fraction 15A-1B). The protective antigen was purified further by detergent (Emulphogene BC720) treatment and adsorption to aluminum hydroxide gel (designated fraction 15A-108A). Compared with B. pertussis vaccine and fraction 15A-1B, fraction 15A-108A retained protective activity as assessed by the mouse protection test, but had reduced protein and markedly reduced endotoxin content. Fraction 15A-108A also had reduced leukocytosis-promoting, histamine sensitizing splenomegaly-inducing, and adjuvant activities. Emulphogene treatment provided a relatively simple method for removing endotoxin from a potential acellular B. pertussis vaccine. |
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