Batch fermentations on synthetic mixed sugar and starch medium with amylolytic lactic acid bacteria |
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Authors: | Mette Hedegaard Thomsen Jean Pierre Guyot Pauli Kiel |
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Institution: | Plant Research Department, Risoe National Laboratory, BIO-NRG-Building 301, P.O. Box 49, 4000, Roskilde, Denmark. mette.hedegaard.thomsen@risoe.dk |
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Abstract: | The green crop drying industry in Denmark uses Italian rye grass, clover, and alfalfa as raw materials for the production
of green pellets. The green crop drying industry solves its energy economical problems by heating and pressing of the green
crop before drying. The produced sidestream is called brown juice. Brown juice was shown to be an excellent medium for lactic
acid fermentation. The aim of this study was to investigate the utilisation of brown juice in the production of polylactic
acid, where wheat starch would be added to increase the lactic acid yield and, thus, the feasibility of the process. A number
of amylolytic lactic acid bacteria have been identified, and in this work, six different strains were tested for their ability
to produce α-amylase and to utilise all sugars with high lactic acid yield in a medium with a complex composition of free
sugars (brown juice) and starch. Lactobacillus plantarum A6 was the only strain that showed both a good lactic acid production and utilisation of starch in this medium. The growth
rate of this strain was approximately 0.4 h−1 and the lactic acid yield was 0.7. |
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Keywords: | Amylolytic lactic acid bacteria Brown juice Starch Polylactic acid |
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