Functional conservation of the Salmonella and Shigella effectors of entry into epithelial cells |
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Authors: | Daniel Hermant,Robert Mé nard,Nathalie Arricau,Claude Parsot, Michel Y. Popoff |
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Affiliation: | Unitédes Entérobactéries et UnitéINSERM 389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France; Unitéde Pathogénie Microbienne Moléculaire et UnitéINSERM 389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France |
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Abstract: | A Salmonella typhi chromosomal locus composed of five adjacent genes, designated sipEBCDA , was identified by transposon mutagenesis as being essential for cell invasion. Products of the sip genes exhibit extensive sequence similarities to the effectors of Shigella entry into epithelial cells encoded by the virulence plasmid-borne ipa operon. Expression of sipE and sipB in a Shigella non-invasive ipaB mutant restored the ability to invade epithelial cells. The structural and functional conservation of the Sip and Ipa proteins suggests that Salmonella and Shigella entry processes are promoted by similar effectors. |
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