Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium |
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| Authors: | J Nam K S Mysore C Zheng M K Knue A G Matthysse and S B Gelvin |
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| Institution: | (1) Department of Biological Sciences, Purdue University West Lafayette, IN 47907-1392, USA e-mail: gelvin@bilbo.bio.purdue.edu Tel.: +1-765-4944939; Fax: +1-765-4961496, US;(2) Department of Biology, University of North Carolina Chapel Hill, NC 27599-3280, USA, US |
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| Abstract: | We have identified T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium tumefaciens (rat mutants). These mutants are highly recalcitrant to the induction of both crown gall tumors and phosphinothricin-resistant
calli. The results of transient GUS (β-glucuronidase) assays suggest that some of these mutants are blocked at an early step
in the Agrobacterium-mediated transformation process, whereas others are blocked at a step subsequent to translocation of T-DNA into the nucleus.
Attachment of Agrobacterium to roots of the mutants rat1 and rat3 was decreased under various incubation conditions. In most mutants, the transformation-deficient phenotype co-segregated
with the kanamycin resistance encoded by the mutagenizing T-DNA. In crosses with susceptible wild-type plants, the resistance
phenotype of many of these mutants segregated either as a semi-dominant or dominant trait.
Received: 26 October 1998 / Accepted: 8 January 1999 |
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| Keywords: | Agrobacterium Arabidopsis Plant transformation T-DNA |
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